Natural scienceBioinformaticsDNA sequencingIllumina

Limitations and errors of the Illumina method

Read the read

Report a typo

Why adapters can sometimes get sequenced too?

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The length of a read is shorter than the sequenced fragment

It always happens to 1% of total reads, no certain reason

The fluorescent markers got mixed in Illumina machine

The length of a read is longer than the sequenced fragment

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