Natural scienceBioinformaticsDNA sequencingIllumina

Limitations and errors of the Illumina method

Homopolymers

Report a typo

What can we do about homopolymers errors? Is it possible to fix?

Select one or more options from the list

We can remove homopolymers from the non-coding regions of the sequence

There is a special software for realigning reads and fixing the issue

We can cross-validate the sequence, do another run with another machine

Unfortunately, this is impossible to fix. We should rerun the experiment as it's a technical issue

___

Create a free account to access the full topic