Natural scienceBioinformaticsDNA sequencingIllumina

Limitations and errors of the Illumina method

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_________ occurs when a nucleotide's blocker is not properly removed after signal detection. The old nucleotide is detected once more in the following cycle because no new nucleotide can bind to this fragment of DNA. This DNA fragment will now be 1 cycle out of phase with the other DNA fragments, _________. Such errors contaminate the light signal that the sequencer's camera should read.

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