Natural scienceBioinformaticsDNA sequencingIllumina

Illumina quality control

Reads content

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The following picture represents forward and reverse reads from one spot. Technical sequences are highlighted gray, other bases belong to DNA insert. What is true about this sequence pair?

Forward and reverse reads from the same spot

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Insert size is smaller than read length, that's why we observe technical sequence on 3'-end

Reverse read contains unique information compared to forward read

Reverse read duplicates information contained in forward read

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