Natural scienceBioinformaticsBioinformatics algorithmsAlignment algorithms

Introduction to BLAST

E-value intuition

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You get an E-value = 10. How can the result be explained in simple terms?

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10 random shuffling of the sequence database is needed to find 1 alignment with as good score as we observed in the alignment

In 1 database random shuffling we expect 10 alignments with as good scores or better as in observed alignment

E-value can’t be 10 because the probability cannot be greater than 1

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