Natural scienceBioinformaticsData and Tools

SAM & BAM formats

FLAG field

Report a typo

You mapped reads against reference genome and discovered some "0" values in the second field of SAM file. How would you interpret it?

An overview of the different properties that can be encoded in the FLAG field

Select one or more options from the list

Mapping was unsuccessful

We can't see 0 in the second field

This read is unpaired

This read was successfully mapped to the reference, in the forward direction

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