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Introduction to immunoassay technology

Theory

Lab Time

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Put competitive ELISA protocol steps in the correct order.

Put the items in the correct order
Wash off primary antibody-analyse complexes
Add secondary, capture antibodies to the solution
Incubate primary antibody-analyte complexes solution with the reference antigen bound to the plate
Add the substrate and wait for the reaction to happen
Incubate sample with primary antibody
Wash off unbound reference antigen
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