Natural scienceBioinformaticsDNA sequencingIllumina

Illumina/Solexa sequencing method

Principle of illumina sequencing:

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during the extension step four dNTP solutions (dATP, dTTP, dGTP and dCTP) are added one after another untill the correct letter is binded to the growing chain

every nucleotide recognition is followed by the wash step in which the modification that prevents further synthesis is cleaved

every nucleotide recognition is followed by the wash step in which its fluorophore is cleaved

each nucleotide of the template is recognized with its own unique fluorescent signal

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