Natural scienceBioinformaticsBioinformatics algorithmsAlignment algorithms

Running BLAST

Application

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What is true about the following alignment?

Local alignment of short query sequence: Score, E-value and other metrics are shown

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The hit is significant if we choose 0.05 significance threshold

The hit is insignificant if we choose 0.05 significance threshold

Only a short stretch of the subject is aligned to the query

Subject sequence is 588 nucleotides long

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