Natural scienceBioinformaticsData and Tools

FASTA, FASTQ

All About That Base

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If the Phred Quality Score is 40, what does it mean?

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It means that the probability of incorrect base call is 1 in 10000 (and it's bad).

It means that we have a base call accuracy of 99.99% for that nucleotide.

It means that the probability of incorrect base call is 1 in 10000 (and it's good).

It means that we have a base call accuracy of 99.999% for that nucleotide.

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